Stimulatory effect of IL-1 secreted by fibroblasts taken from periodontitis lesion
Dr. Mohammed Wael Daboul, D.D.S, MS.c Biology US. , MT-ASCP, US., Laboratory Medicine Specialist.
Interlukin 1 is an inflammatory factor which has a local and generalized effect by its secretory
type through the circulation. Fibroblasts, on the other hand , are considered of great
importance in connective tissue metabolism including periodontal tissues . They secret
interlukin 1 with other additional substances .
The purpose of our study was to isolate these fibroblasts taken from periodontitis location and
from normal periodontal tissues and grow them in vitro. Then to compare the secretion of
Interlukin 1 from fibroblasts obtained from the normal source and the periodontal disease
source .If difference is found, this will support the theory of the presence of subpopulation of
fibroblasts that have different functional parameters that lead to changes in the tissue matrix
which clinically manifests as periodontitis.
Fibroblast are isolated from normal and abnormal periodontal tissues and then grown in
suitable media , then The fibroblast metabolites are harvested and added to the thymocytes
which are incubated with radio active thymidine.
Results showed that radioactive Thymidin taking cells count after two days of incubation with
the metabolites from periodontitis source was 2920 +/- 185 and after four days it became 5500
+/- 227 , while the count after two days of incubation with the metabolites from fibroblast
obtained from normal periodontal tissues was 886+/-142and 1239 +/- 133 after four days of
incubation respectively . The difference is considered in accordance with the student T test a
significant one . This supports the suggestion that gingival connective tissue pathogenesis
involves not cellular injury but selection , by unique conditions existing within the affected
tissue, of unusual fibroblast subpopulation characterized by functional parameters that lead to
changes in the tissue matrix .
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